АС UTE VIR AL HEPATITIS B (VHB) F. Hepadnaviridae, G. Orthohepadnavirus. Virus презентация

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Презентации» Образование» АС UTE VIR AL HEPATITIS B (VHB) F. Hepadnaviridae, G. Orthohepadnavirus. Virus
АСUTE VIRAL HEPATITIS B (VHB)
  F. Hepadnaviridae, G. Orthohepadnavirus.
 All these proteins are found out in plasma during replication ofAgainst all antigenes the antibodies are worked out 
  majorEPIDEMIOLODY:
  33 % of the population of a planet transferFrequency of revealing of markers VHB:
  - Among medical workers2. Artificial of modes of transmission:
   - all medicalPATHOGENY
 1. Implantation of a virus after a sorption on surface
3. The assembling of viruses is completed by a secretion 
6. The immune system of the host destroys:
  - hepatocytes,9. Except for immune cytolisis hepatocytes damage:
  - antibody -FREQUENCY of SIGNS of INITIAL PERIOD
 
  - Lowering appetiteWith appearance of an icterus - intoxication intensify:
  - aFULMINANT the FORM 
   Results to acute hepatic unsufficiencyAppearance encephalopathy with an oppression of consciousness, degree expressivenesses which is3. Verbal is oriented
       CLINICAL CRITERIA VHB:
 
  - average age of the patientsA 16-year-old Caucasian male born of a hepatitis B carrier motherA serologic profile to screen for hepatitis B virus infection
 VIRAL HEPATITIS Д
  The agent – RNA-virus by a sizeCLINIC. Incubation interval 80 days (40 - 200 days)
  CoinfectionThe severe current is registered for 60 % of the patients- pain in the right upper quadrant in initial period
 On a surface are posed glycoproteins Е. 6 genotypes of aCLINICAL CRITERIA VHC:
  - average age of the patients
 A 69 - year-old female presents with fatigue, arthragias, peripheral paraesthesis,1. A 48 – year-old male developed an attack of anginaTREATMENT
 
 The mild forms - bed rest, diet N5, hepatoprotections,Hemosorption, enterosorbents , streptomycin 2- 4 gr PO, drugs of aFor unloading a small circle of a circulation - lasixum, euphyllinum,1. A 48 – year-old male developed an attack of angina2
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АСUTE VIRAL HEPATITIS B (VHB) F. Hepadnaviridae, G. Orthohepadnavirus. Virus has the spherical form by a size 42 nm, is covered lipoproteinal envelope with the thickness by 7 nm (It is HBs Ag). The nucleocapsid (соre) has a diameter by 28 nm inside contains ring two-filamentous DNA. Internal (+) chain on 25 - 40 % more shortly outside (-) chain. Genome of VHB contains S, C, X, P genes. The formation of main protein and his subtypes encodes by genes S, pre-S1,pre-S2. The protein pre-S2 has specific receptors to albumin, which allocates on hepatocytes The protein S 1 - carrier of a dominating antigenic site "a" also answers for species specificity of a virus.


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All these proteins are found out in plasma during replication of viruses!!! The gene C- encodes protein of a nucleocapsid HBcAg (it is only inside hepatocytes), but his soluble antigenic variety HBe Ag circulates in a blood and has 3 serovars Gene Х - the activator of all genes VHB, probably induces appearance of a hepatocell carcinoma (these patients have for high number anti-HBX!) The gene Р - occupies 80 % (-) chain DNA - encodes DNA-polymerase, return transcriptase, ribonuclease The surface antigene НBsAg ( size from 22 up to 700 nm) has 4 main subtypes (ayw, ayr, adw, adr)

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Against all antigenes the antibodies are worked out major markers of this hepatitis. Cytopathic of effect virus - DOES NOT RENDER!!! Is very steady in the environment, being inactivated: - At room temperature - in 6 months - At 60 dg. C- in 10 hours - At 98 dg. C- in 20 minutes - At 160 dg. C (dry fever) - in 1 hour - In an autoclave (120 dg. С) - in 30 minutes - In the dried up plasma - in 25 YEARS!!! - In a refrigerator - in 6 months - At рН 2.4 - in 2 hours - In usual concentrations of disinfectant solutions - through 2 - 7 days!!!

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EPIDEMIOLODY: 33 % of the population of a planet transfer VHB and 350 - 400 millions from them have remained the carriers HBsAg!! Groups of high risk - Мedical workers, contacting with a blood - Recipients of biological tissues of the man - Patients with often parenteral infusions - Mentally retarded and looking after staff - Patients with chronic diseases of a liver - Children birthing from the mothers - carriers HBsAg - Drug addicts and prostitute - Homosexuals and persons by HIV-infection - Inhabitants hyperendemic on VHB of districts of a earth

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Frequency of revealing of markers VHB: - Among medical workers - 33.3 % - Among the patients with venereal diseases – 49.3 % - Among the donors of a blood - 14.5 % - Among the population - 3.8 % The mode of transmission - PARENTERAL. Natural modes of transmission: - sexual - 51.9 % - intranatal and postnatal - 1% - 10% - 50 % - transplacental - 6 - 10 % - hemopercutaneus (home contacts) - 6 - 10 %

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2. Artificial of modes of transmission: - all medical manipulations with damage skins and mucous - IV drug addicts , tattoo, cosmetic procedures Infection a dose of a blood - 0,00004 - 0,000001 ml. Frequency infection newborn – 0.02 - 40 % The hazard of a chronic carriage HBsAg after infection: - at infection newborn - 90 %, - 1-st year of life - 50 %, - is higher than 1-st year 20 % - and for adult - 5 - 10 % The one injection of a blood with HBsAg - causes diseases in 90 % of cases. Hazard after a one prick of infection needle - 34 % In 50 % of cases the source of an infection is not revealed!

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PATHOGENY 1. Implantation of a virus after a sorption on surface of a hepatocyte through protein pre- S2 by the method endocytosis. 2. "«Undressing" of a virus and beginning of his replication: - the nucleocapsid is shaped in core of hepatocyte, where through virus DNA-polymerase the chain (+) is completed !! - is shaped m - RNA (with the help cellular RNA-poly- merase on which there is a copying at once (-), and then and (+) chains - synthesis of protein of the external envelope occurs in cytoplasma

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3. The assembling of viruses is completed by a secretion in a blood soluble nucleocapsid HBeAg, which decrease both cellular (lowering of production Inter- ferons) and humoral (suppression formation antibody and induction immunotolerance), which impedes his going out from hepatocytes 4. Except for liver replication of a virus is possible in cells of an marrow brain, spleen, monocytes and of lymphatic tissue. 5. The immune answer at VHB corresponds immuno- pathological response - clinical form arise at parti- cular activity Т-cellular immunity. When this immunity depressed completely - the disease does not develop!!!

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6. The immune system of the host destroys: - hepatocytes, containing VHcAg, - damages cells with protein pre-S2 - but is neutral to cells with HBsAg!!! 7. Humoral immunity formation of an antibody In the following sequence: - anti - HBc Ag Ig M, then IgG - anti -pol and anti -pre S1 - anti -HBe Ag and anti -pre S2 - anti -HBs Ag (occur last) - anti -HBxAg (are not reveald for all patients) 8. Can occur circulating immune complexes especial at shape of the chronic forms hepatitis!!!

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9. Except for immune cytolisis hepatocytes damage: - antibody - dependence immune cytolisis of hepato- cytes, on surface which the complex antigene- antibody is shaped what increased activity by Tk – lymphocytes - autoantibodies against of a hepatic lipoprotein - violation of a metabolism in the struck cells CLINIC VHB Incubation 80 days (from 40 about 200 days) More often the patients have of average age (18 - 55 years) Positive parenteral anamnesis or contact to carriers HBs Ag Step-by-step beginning of disease with long-lived by preicteric period

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FREQUENCY of SIGNS of INITIAL PERIOD - Lowering appetite 95,6 (in %) - Weakness 94,5 - Nausea 65,3 - Gravity in the right upper quadrant 57,2 - Joint pain, muscles pain 46,8 - Headache 34 - Meteorism 32,9 - Vomiting 31,5 - Abdominal pain 28,4 - Fever 22,5 - Dermal itch 21,2 - Diarrhea 9,5 - Giddiness 8,2 - Dermal eruption 8

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With appearance of an icterus - intoxication intensify: - a nausea, vomiting, anorexia - icteric colouring of a skin and scleras, dark urine, decolorized feces, - increase of a liver and lien for all patients. VHB more often proceeds in the moderate form, but can be both mild, and severe forms of disease . Fulminant the form up to 2 % Duration of icteric period from 1 about 12 weeks. Cholestatic of the form of illness from 5 up to 15 % Lingering current from 5 up to 10 % The period of a reconvalescence can be delayed on some months

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FULMINANT the FORM Results to acute hepatic unsufficiency and lasts from 2 about 8 weeks, being ended in 80 - 90 % of cases by death of the patients. For it are characteristic: - obstinate nausea and vomiting, anorexia - progressing hemorrhagic syndrome - fast decrease of sizes and density of a liver - a "hepatic" smell of a skin and exhaled air - a neutrophilic leukocytosis - accelerated ESR - decrease АLТ and АSТ, prothrombin Index - Increase of a fraction of an indirect bilirubin - increase of prothrombin time more 12 cek.

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Appearance encephalopathy with an oppression of consciousness, degree expressivenesses which is estimated in marks on Glasgow scale : 1 Opening spontaneous 4 of an eyes in reply to the verbal order 3 In reply to pain stimulation 2 absence 1 2. Active move- spontaneus and reply in the order 6 ments single-minded on pain 5 unsingle-minded on pain 3 pathological tonical flexion to pain 3 pathological tonical extension to pain 2 absence of a motion to pain stimulation 1

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3. Verbal is oriented Fast answers - 5 The answers the confused speech - 4 The inadequate answers - 3 Inarticulate sound - 2 Absence of speech - 1 15 - marks - norm, 13 - 14 marks - somnolencea 9-12 marks - sopor, 4-8 marks - coma, 4 marks and less - out-of-limit coma.

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CLINICAL CRITERIA VHB: - average age of the patients or children till 1 year - positive parenteral anamnesis, - contact to the carrier HBs Ag or group of hazard - step-by-step beginning of disease - long-lived initial period with arthralgias and by allergies - aggravation of symptoms after appearance of an icterus - long-lived current with transition in chronic the forms in 5 - 10 % of cases - fulminant current up to 2 % of cases - revealing in a blood of markers VHB (ELISA) - revealing in a blood of DNA VHB (PCR)

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A 16-year-old Caucasian male born of a hepatitis B carrier mother was incidentlly discovered to have serum HBsAg and elevated ALT are AST. He denied any symptoms. His liver was not palpable. Six months later he still had elevated ALT values. Abdominal ultrasound examination reavealed a normal liver and spleen. The needle biopsy shows preservation of the lobular architecture. The portal area has moderate degree of chronic inflammation with mononuclear cells.There is mild piecemeal necrosis, the parenchyma shows foci of necrosis, and several hepatosytes have uniformly pale eosinophilic cytoplasm (ground glass cytoplasm ) indicating intracellular accumulation of HBsAg. Laboratory investigations: ALT- 312 U/L , AST- 164 U/L, Platelet count - 250 x 10 in 9 dg., Bilirubin – 18.8 mmol/L, Albumin – 43 g/L, HBV-DNA > 1,000 pg/mL., anti-HBc IgM –absent, HBsAg - present, HBeAg – present, anti-HCV – absent

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A serologic profile to screen for hepatitis B virus infection HBsAg antiABs antiHBc antiHBc HBeAg antiHBe HBV-DNA IgG IgM A. - + + - - + - B. + - + - - + - C. - + - - - - - D. + - - + + - + E. + - + - - + + A. Previous infection with immunity B. Chronic hepatitis B ( wild type) C. Previous hepatitis B vaccination D. Acute hepatitis B E Chronic hepatitis B ( precore mutant)

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VIRAL HEPATITIS Д The agent – RNA-virus by a size 28 - 39 nm with the envelope consisting from HBs Ag. RNA of the virus is surrounded HDV core Ag. In a blood of the patients transmute in rodshaped inbranched the form. Is steady against warming, operation of acids and proteinases, but fails by alkalis and nucleases. HDV - is selected only from the patients infection HBV. The monoinfection VHD is impossible!!! Can cause disease only in a combination with VHB as coinfection or superinfection EPIDEMIOLOGY 15 million of carriers VHD are in the world. It is revealed: among carriers HBs Ag – in 9 % of cases and among the patients with chronic VH - in 10 - 30 % of cases

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CLINIC. Incubation interval 80 days (40 - 200 days) Coinfection - simultaneous infection HBV and HDV. Clinical manifestations of VHD differs from a VHB little, but more often: - high temperature from 3 to 5 days and long-lived 37,2 – 37,8 dg.С the one registers . - more often complain of a pain in the right upper quadrant - characteristicly two-undular current with peaking on 15 – 32 days of illness. It more often revials among the recipients of a blood and drug addicts. Transform to a chronic hepatitis in 5 % of cases The superinfection develops for carriers HBsAg After repeated hemotrasfusion and infection result - aggravate a hepatitis with intensive reproduction of a virus in hepatocytes.

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The severe current is registered for 60 % of the patients with creation fulminant of the forms of disease. More than 70 % becomes chronic carriers HDV, Proceeding more hardly, than at VHB (frequently with a hydropic - ascitic syndrome) Passes in a cirrhosis for 60-70 % of the patients!! CLINICAL CRITERIA VHD: - Average age of the patients - Hemotransfusion or carriage HBs Ag - Acute beginning, short preicteric period with arthralgias and allergies - Aggravation of symptoms of the patients about appearance icteruses

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- pain in the right upper quadrant in initial period - hepatospleenomegalia - high and long-lived hyperbilirubinemia - appearance of a hydropic - ascitic syndrome at superinfections - clinico-enzymatic peakings at coinfection - severe current for 60 % of the patients and often outcomes In a chronic hepatitis for 70 % at a superinfection VIRAL HEPATITIS С: The agent - cover spherical virus, size 35-50 нм. (F. Togaviridae, G. Flavivirus.), Inside is one-filamentous RNA (+), is covered by protein C.

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On a surface are posed glycoproteins Е. 6 genotypes of a virus are known. Is sensitive by fatty-solvent, 60 dg С maintains - 30 minutes, boiling - 2 minutes EPIDEMIOLOGY: - is similar VHB, but is very seldom transmitted upright and sexual way. it is posttrasfusion a hepatitis in 50 -60 %. Mainly is average age of the patients. The data on quantity in the world of carriers is great, but the figures are contradictory (100 - 500 millions of man) CLINIC. An incubation 42 days (14 - 60 days) Beginning step-by-step. Principal syndromes are dyspeptic and astheno-vegetative. With appearance of an icterus the state is aggravated, but is less significant, than at VHB.

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CLINICAL CRITERIA VHC: - average age of the patients - In an anamnesis hemotrasfusions or contact to the ill patient - step-by-step beginning - aggravation of symptoms with appearance of an icterus - moderate increase of a bilirubin and liver - transition in 50 - 80 % of cases in the chronic form with the subsequent flaccid current ( “affectionate” the murderer) - antibodies occurs late, in 5 years of an antibody do not influence to replication of a virus - It are found out of antibodies- ELISA and RNA- PCR

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A 69 - year-old female presents with fatigue, arthragias, peripheral paraesthesis, leg edema, and purpura. On examination, the patient has moderate hypertesion ( 170/110 mmHG ). Purpura involves both extremites and the left hemithorax. The liver and spleen are not palpable. Abdominal ultrasoud shows ascitic fluid around liver and in the pelvis. Chest X-rays show bilateral basal pleural effusions. A liver biopsy shows minimal inflammatory changes. Laboratory investigations: Hemoglobin – 76 g/L, Both ALT and AST- normal, Cryoglobulins present 4.1 g/L:IgG+A+M, Total protein 51 g/L, Albumin 26 g/L, Creatinin 194.5 mm/L, BUN- blood urea nitrogen 11.1 mmol/L, HBsAg absent, Anti-HCV present, Albuminuria 17g/L, Rheumatoid factor positive , Urine showed micro/macro hematuria.

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1. A 48 – year-old male developed an attack of angina for which he underwent a thorough investigation. Biochemical liver tests revealed an ALT of 184 U/L, AST of 104 U/L, and a positive anti – HCV test result. He denied risk factors for hepatitis. His wife was anti-HCV negative but his brother was found to be anti-HCV positive. Both of his parents died of cause unrelated to hepatitis. The liver was not palpable. His abdominal ultrasound was normal. A liver biopsy shown: enlaged portal tracts with predominantly lymphocytic infiltration. Lymphocytes are arranged in a follicle-like structure. The limiting plate is preserved. Bile ducts are slightly elongated. Liver cells are arraged in regular one-cell-thick plates. Laboratory investigations: Bilirubin – 13.68 mmol/L, Prothrombin time – 12.2 seconds, Platelet count 215 x 10 in 9 dg./L, Ferritin – 620 ng/mL, Albumin - 43 g/L, Cryoglobolins – absent, Tissue antibodies – absent, HB SAg – absent, HCV-RNA type 1b - 1.850 x 10 in 6 dg. genomes /mL.

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TREATMENT The mild forms - bed rest, diet N5, hepatoprotections, vitamin therapy, PO desintoxication, antioxidants Moderate forms: therapy as mild form + follow-up amplifies desintoxication, the glucocorticoids (at VHB are more often) The severe forms: - antiviral drugs (interferons, vidarabin, lamivudin,( is rare) - immunodepretion drugs at VHB (antilymphocytic Immunoglobulin, glucocorticoids etc.) - antiferment drugs and antifibrinolytic drugs -Intensifying desintoxication (exception of a diet of a protein)

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Hemosorption, enterosorbents , streptomycin 2- 4 gr PO, drugs of a levulose etc. At encephalopathy – glutamic an acid, glutargin, sodium a hydroxybutyrate) Thrombocytopenia a hemorrhagic syndrome (vitamin C (antiscorbutic factor) and vitamin K ( antihemor -rhagic factor) acidum aminocapronicum, etamsylatum sodium Coagulopathy and hemorrhagic syndrome – at 1st stage DIC heparinum for 1000 U/HOUR IV For decrease of a hypoxia of tissues (oxygenium, calcium pangamat, cytochrome C 10 мг/kg) For improvement of a metabolism (spleninum, riboxinum, orotat of a potassium, cocarboxylase etc.)

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For unloading a small circle of a circulation - lasixum, euphyllinum, sulfocamphocainum, cardiac glycosides At an inefficiency of treatment – makes of a liver grafting PROPHYLAXIS To keep general purpose standards precautions by operation with the biological preparations To test a donor blood, bodies, semen Vaccinal prevention from VHB and VHD (Engerix B etc.) in a dose 20 mkg with an interval 0 - 1 - 6 months Sanitary - educational operation

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1. A 48 – year-old male developed an attack of angina for which he underwent a thorough investigation. Biochemical liver tests revealed an ALT of 184 U/L, AST of 104 U/L, and a positive anti – HCV test result. He denied risk factors for hepatitis. His wife was anti-HCV negative but his brother was found to be anti-HCV positive. Both of his parents died of cause unrelated to hepatitis. The liver was not palpable. His abdominal ultrasound was normal. A liver biopsy shown: enlaged portal tracts with predominantly lymphocytic infiltration. Lymphocytes are arranged in a follicle-like structure. The limiting plate is preserved. Bile ducts are slightly elongated. Liver cells are arraged in regular one-cell-thick plates. Laboratory investigations: Bilirubin – 13.68 mmol/L, Prothrombin time – 12.2 seconds, Platelet count 215 x 10 in 9 dg./L, Ferritin – 620 ng/mL, Albumin - 43 g/L, Cryoglobolins – absent, Tissue antibodies – absent, HB SAg – absent, HCV-RNA type 1b - 1.850 x 10 in 6 dg. genomes /mL. What is the diagnosis? Comment on the virological data? Should this patient be treated?

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